metabolic function of selenium, assessed with rat liver homogenates
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metabolic function of selenium, assessed with rat liver homogenates

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Published .
Written in English


  • Selenium -- Physiological effect.

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Edition Notes

Statementby Richard Charles Bull.
The Physical Object
Pagination81 leaves, bound :
Number of Pages81
ID Numbers
Open LibraryOL14289910M

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A metabolic function of selenium, assessed with rat liver homogenates. By. These liver preparations\ud (either homogenates or mitochondria preparations) were used to study\ud the metabolic activities of selenium and vitamin E in a NAD- and\ud flavoprotein-dependent system, using pyruvate or succinate as the\ud substrate.\ud Results showed. A METABOLIC FUNCTION OF SELENIUM, ASSESSED WITH RAT LIVER HOMOGENATES INTRODUCTION Early studies with selenium were oriented toward the problem of toxicity due to excess in the diet of animals, and it was not until that Schwarz and Foltz discovered that minute quantities of this element could play a useful role in preventing liver necrosis in the rat.   Essentiality and Metabolic Functions of Selenium Klaus Schwarz, M.D. * The discovery that selenium is an essential trace element of pro­found physiological significance in warm-blooded animals was made in This finding, in conjunction with the prior discovery of cobalt as an integral part of vitamin B12, gave a new turn to nutritional by: Protein Catabolism in Rat Liver SlicesWe began our investi- gation by repeating selected portions of the earlier reports that metabolic energy inhibitors inhibited protein catabolism (). Liver slices were prepared, incubated, and analyzed as described by Simpson (6). .

The metabolism of selenium is dynamic and can be likened to in vivo combinatorial chemistry, in the sense that a wide array of products are formed (7, 8). Animals synthesize many different intermediary metabolites in the course of converting inorganic Se to organic forms and vice versa. The homogenates were centrifuged at rpm for 10 min at 4 ºC (MPWR, Korea) and post-mitochondrial supernatant (PMS) was used for the estimation of total protein and lipid peroxidation. Metabolic Functions of the Liver. Hepatocytes are metabolic overachievers in the body. They play critical roles in synthesizing molecules that are utilized elsewhere to support homeostasis, in converting molecules of one type to another, and in regulating energy balances.   The overall antioxidant status in hepatocyte homogenates immediately after isolation was not significantly different from that of liver homogenates ().After attachment to collagen, 4 h after seeding, lipid peroxidation, as assessed by the concentration of TBARS, as well as GSH content, GST, GSSG-R and GSH-Px activities, were not significantly different compared to liver homogenates and.

by Rat Liver Homogenates mg. of rat liver homogenate (unfasted rat), ml. of M KgHPOa, pH ~ ml. of M fumarate, ml. of rat muscle extract, y of dye in ml. of ethanol, and water to a final volume of ml. Incubated for 30 minutes. Factors added. Selenium (Se) is an essential micronutrient involved in important metabolic and physiological functions in all vertebrate species. Se is known to have a narrow margin between essentiality and toxicity, particularly in oviparous vertebrates such as fishes (Janz etal., ).Under normal physiological conditions, fish require dietary concentrations of to μg Se/g dry mass (d.m.) to. The role of selenium in biological systems have been investigated over the years to demonstrate the effects of selenium, for example, it helps to promote a healthy liver, acts as an antioxidant [5. Part of the Biochemistry of the Elements book series (BOTE, volume 2) K., Essentiality and metabolic functions of selenium, Med. Clin. No. Am. L. M., Prevention of decline of α-ketoglutarate and succinate oxidation in vitamin-E-deficient rat liver homogenates, J. Biol. Chem. – PubMed Google.